Identification of left-handed Z-DNA by indirect immunofluorescence in polytene chromosomes of Chironomus thummi thummi |
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| Authors: | F Lemeunier C Derbin B Malfoy M Leng E Taillandier |
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| Institution: | 1. Biologie et Génétique Evolutives, CNRS, F-91190 Gif sur Yvette, France;2. UER de Médecine et de Biologie Humaine, Université Paris XIII, F-93012 Bobigny Cedex France;3. Centre de Biophysique Moléculaire, CNRS, F-45045 Orleans Cedex, France |
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| Abstract: | In this work, antibodies against Z-DNA were used to stain polytene chromosomes of Chironomus thummi thummi. By indirect immunofluorescence we report the first identification of left-handed conformation of DNA in a band region. The Chironomus pattern also contrasts with the general staining observed in Drosophila. In Chironomus the antibodies to Z-DNA bind to one interband region of the chromosome II and two bands regions of the chromosome IV. |
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| Keywords: | To whom offprint requests should be addressed |
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