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The role of Src protein in the process formation of PC12 cells induced by the proteasome inhibitor MG-132
Authors:Oktá  via Tarjá  nyi,Gergely Berta,Alexandra Harci,Eszter B. Bacsa,Borbá  la Stark,Marianna Pap,Jó  zsef Szeberé  nyi,Gyö  rgy Sé       Jr.
Affiliation:1. Department of Medical Biology, Medical School, University of Pécs, Pécs, Hungary, Szigeti út 12, H-7643 Pécs, Hungary;2. Signal Transduction Research Group, János Szentágothai Research Centre, Pécs, Hungary, Ifjúság útja 20, H-7624 Pécs, Hungary
Abstract:
Keywords:Cy3,    a fluorophor cyanin dye   DMEM,    Dulbecco&rsquo  s Modified Eagle&rsquo  s Medium   DMSO,    dimethyl-sulfoxyde   EDTA,    ethylenediaminetetraacetic acid   EGTA,    ethylene glycol tetraacetic acid   ERK1/2,    extracellular signal-regulated kinase 1 and 2   H-Ras,    an isoform of the small guanine nucleotide-binding protein Ras named after Harvey   IκB,    inhibitor of kappa B   JNK,    c-Jun N-terminal kinase   MAP Kinase,    mitogen-activated protein kinase   MEK,    mitogen-activated protein kinase/ERK kinase   MG-132,    a peptidyl-aldehyde proteasome inhibitor, also known as Z-Leu-Leu-Leu-al   M-M17-26,    a variant PC12 clone that stably expresses a dominant negative H-Ras mutant   NGF,    nerve growth factor   nnr5,    a TrkA-deficient PC12 variant clone   p38MAPK,    a MAP kinase with the molecular weight of 38   kDa   PBS,    phosphate buffered saline   PC12,    rat pheochromocytoma cell line   PD98059,    a chemical compound that inhibits MEK   PMSF,    phenylmethanesulfonyl fluoride   PP2,    4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine, an inhibitor of Src   PVDF,    polyvinylidene difluoride   Ras,    a monomeric G-protein   Src,    a non-receptor tyrosine kinase   SDS,    sodium dodecyl sulfate   TBS,    Tris-buffered saline   TrkA,    tropomyosin-related kinase
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