| 重组[B18Ile]人胰岛素的鉴定和特征 |
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| 引用本文： | 刘滨 唐月华. 重组[B18Ile]人胰岛素的鉴定和特征[J]. Acta biochimica et biophysica Sinica, 1997, 29(4): 409-413 |
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| 作者姓名： | 刘滨 唐月华 |
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| 作者单位： | 中国科学院上海生物化学研究所 |
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| 摘 要： | 突变体「Ｂ１８Ｉｌｅ」猪胰岛素前体经分离纯化，转肽，得到重组「Ｂ１８Ｉｌｅ」人胰素「Ｂ１８Ｉｌｅ」人胰岛素能结晶，其与受体的结合能力为猪胰岛素的８２％，保留了与猪胰岛素基本相同的体内活力，从本文结果和分析表明Ｂ１８Ｖａｌ可能不是胰岛素表现生物功能所必需的。
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| 关 键 词： | 胰岛素 结构 功能 重组 鉴定 |
Expression and Activity of Recombinant Human Augmenter of Liver Regeneration |
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| YANG Xiao Ming,XIE Ling,HE Hao,WEI Han Dong WU Zu Ze and HE Fu Chu. Expression and Activity of Recombinant Human Augmenter of Liver Regeneration[J]. Acta biochimica et biophysica Sinica, 1997, 29(4): 409-413 |
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| Authors: | YANG Xiao Ming XIE Ling HE Hao WEI Han Dong WU Zu Ze HE Fu Chu |
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| Abstract: | The cDNA of human augmenter of liver regeneration was subcloned onto the downstream of the P RP L promoter of the expression plasmid pBV220. The recombinant plasmid could stabelly express ALR with high efficiency (up to 20% of the total bacterial proteins) in E.coli through thermal induction.The expressed recombinant ALRs could produce a significant increase in the incorporation of 3 H TdR into liver DNA of a 1/3 hepatectomized test animal and also had a potent antihepatitis effect. These results suggest that ALR appears to be an important regulator of liver regeneration and may be used in clinical trial for enhancing liver regeneration in the treatment of hepatic diseases. |
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| Keywords: | Augmenter of liver regeneration Liver regeneration Expression DNA synthesis |
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