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Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer |
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| Authors: | Kwang Sung Ahn Ji Young Won Jin-Ki Park Alice M. Sorrell Soon Young Heo Jae-Seok Woo Won-Kyong Chang |
| Affiliation: | a Department of Physiology, Dankook University School of Medicine, Cheonan, Republic of Korea b Animal Biotechnology Division, National Institute of Animal Science, Suwon, Republic of Korea c Department of Nanobiomedical Science, Dankook University, Cheonan, Republic of Korea d Genomics and Bioinformatics Division, National Institute of Animal Science, Suwon, Republic of Korea e Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea |
| Abstract: | This study was performed to produce transgenic pigs expressing the human complement regulatory protein CD59 (hCD59) using the nuclear transfer (NT) of embryonic germ (EG) cells, which are undifferentiated stem cells derived from primordial germ cells. Because EG cells can be cultured indefinitely in an undifferentiated state, they may provide an inexhaustible source of nuclear donor cells for NT to produce transgenic pigs. A total of 1980 NT embryos derived from hCD59-transgenic EG cells were transferred to ten recipients, resulting in the birth of fifteen piglets from three pregnancies. Among these offspring, ten were alive without overt health problems. Based on PCR analysis, all fifteen piglets were confirmed as hCD59 transgenic. The expression of the hCD59 transgene in the ten living piglets was verified by RT-PCR. Western analysis showed the expression of the hCD59 protein in four of the ten RT-PCR-positive piglets. These results demonstrate that hCD59-transgenic pigs could effectively be produced by EG cell NT and that such transgenic pigs may be used as organ donors in pig-to-human xenotransplantation. |
| Keywords: | Nuclear transfer Embryonic germ cell Human CD59 Transgenic pig |
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