Plasma low-molecular-weight thiol/disulphide homeostasis as an early indicator of global and focal cerebral ischaemia |
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| Authors: | Alexander Vladimirovich Ivanov Valery Vasil’evich Alexandrin Alexander Alexandrovich Paltsyn Ksenya Alexandrovna Nikiforova Edward Danielevich Virus Boris Petrovich Luzyanin |
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| Institution: | Department of Molecular and Cell Pathophysiology, Federal State Budgetary Scientific Institution ‘Institute of General Pathology and Pathophysiology’, Moscow, Russia |
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| Abstract: | Objective: Recent studies have shown that cerebral ischaemia causes not only local, but also systemic oxidative stress. This leads to oxidation of thiol-containing compounds, including low-molecular-weight thiols (cysteine, glutathione, homocysteine and others). Therefore, the aim of this work was to verify the hypothesis that the thiol/disulphide homeostasis of low-molecular-weight thiols is disturbed in the early stages of cerebral ischaemia.Methods: Two experimental rat models of ischaemia were used: a global model of vascular ischaemia (clamping the common carotid arteries?+?haemorrhage) and focal ischaemia (middle cerebral artery occlusion). The total levels of thiols and their reduced forms were measured before surgery and after 40 minutes of reperfusion (global) or 3?hours (focal) ischaemia.Results: The global ischaemia model caused a marked (2.5–4 times, P?P?Discussion: These results suggest that plasma low-molecular-weight thiols are actively involved in oxidation reactions at early stages of cerebral ischaemia; therefore, their reduced forms or redox state may serve as a sensitive indicator of acute cerebrovascular insufficiency. |
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| Keywords: | Cerebral ischaemia cysteine glutathione homocysteine oxidative stress rat |
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