Production and Properties of Lipase from a Newly Isolated Chromobacterium |
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Authors: | Tsutomu Yamaguchi Noriyuki Muroya Masakazu Isobe Mamoru Sugiura |
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Institution: | Research Laboratory, Toyo Jozo Co., Ltd., Ohito-cho, Shizuoka-ken |
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Abstract: | Out of some 750 strains of microorganisms, a potent bacterium for lipase production was isolated from soil and was identified as Chromobacterium viscosum.The bacterium accumulates lipase in culture fluid when grown aerobically at 26°C for 3 days in a medium composed of soluble starch, soy bean meal, lard and inorganic salts.Chromobacterium lipase had an optimum pH of 7.0 for activity at 37°C, and an optimal temperature of 65°C at pH 7.0. The enzyme retained 80% of the activity when heated for 10 min at 70°C. This lipase was capable of hydrolyzing a variety of natural fats and oils, and it was more active on lard and butter than on olive oil. The activity was stimulated by Ca2+, Mg2+, Mn2+ and inhibited by Cu2+, Hg2+ and Sn2+. It was not diminished but rather stimulated by a high concentration of bile-salts. |
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Keywords: | cytotoxicity sponge Dysidea marine natural product steroid |
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