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Search for Microorganisms Producing Cephalosporin Acylase and Enzymatic Synthesis of Cephalosporins
Authors:Mikio Shimizu  Taketoshi Masuike  Hironori Fujita  Kazuo Kimura  Ryo Okachi  Takashi Nara
Affiliation:Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo, Japan
Abstract:
Microorganisms were tested for production of cephalosporin acylase. Some bacteria showed strong acylase activity for all of cephalexin, cephaloridine, cephalotin, penicillin G and ampicillin. Some showed a rather specific activity for cephalexin. Pseudomonas melanogenum KY 3987 showed specific activity only for cephalexin and ampicillin which contain a side chain of d-phenylglycine. Most of these acylase-producing bacteria had the ability to synthesize cephalexin and other cephalosporins from 7-aminocephem compounds and organic acid esters. Among them, Ktuyvera citrophila KY 7844 was one of the most promising organisms for enzymatic synthesis of cephalosporins. This organism had the ability to catalyze N-acylation of 7-aminocephem compound not only with α-amino acid ester, but also with such acid esters as 1-(1 H)-tetrazolylacetate methylester which has no α-amino group.
Keywords:Sophora extract  blood glucose level  disaccharidase inhibition  sucrase  oral sugar tolerance test
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