A Convenient Method for the Quantitative Determination of Pipecolic Acid |
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| Authors: | Ryoji Onodera Makoto Kandatsu |
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| Institution: | 1. Department of Agricultural Chemistry, Miyazaki University, Miyazaki, Japan;2. Azabu Veterinary College, Fuchinobe, Sagamihara-shi, Japan |
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| Abstract: | The quantitative determination of pipecolic acid was examined.The reaction of 3% ninhydrin solution in n-butanol, saturated with citrate buffer (pH 4.2), with pipecolic acid in boiling water for 3 min yielded the colored products showing λmax at 570 mμ, but with proline hardly yielded those products. By the colorimetry proposed, it is possible to determine the amount of pipecolic acid in the sample containing proline no more than 50 times the amount of the pipecolic acid, directly from the calibration curve using pipecolic acid.The method for removal of amino acids from the sample containing pipecolic acid and proline was examined and discussed. |
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| Keywords: | biodegradation carbazole heterocyclic nitrogen compound resting cell reaction Sphingomonas sp |
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