Studies on Fungal Tannase |
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Authors: | Hideaki Yamada Osao Adachi Masahiro Watanabe Noriko Sato |
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Institution: | 1. Research Institute for Food Science, Kyoto University, Kyoto;2. Department of Agricultural Chemistry, Kyoto University, Kyoto. |
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Abstract: | The possibility that selective inhibition of phage by antibiotic may be achieved by using bacterial mutant resistant to the antibiotic was investigated in the system of HM-phages of Clostridium saccharoperbutylacetonicum, a butanol-producing bacterium.Consequently, it was found that Oxytetracycline, using the antibiotic-resistant mutant as host, inhibited selectively the growth of HM-phages. The bacterial mutant termed type A (one-step mutant resistant to 30 μg/ml of Oxytetracycline) did not permit the growth of HM-phages (HM 2 and HM 3) in the presence of the antibiotic (ca. 10 μg/ml), though it permitted the growth of the phages in the absence of the antibiotic.An analysis of the mode of action of Oxytetracycline in HM 2-phage system revealed the following, (i) The antibiotic had a slight phagicidal action, (ii) It did not prevent the phage adsorption, (iii) It inhibited the protein synthesis in phage-infected cells, (iv) It inhibited the lysis of infected cells. Active phages were, however, not detected when the lysis-inhibited cells were artificially lysed.Another type of bacterial mutant was also encountered. In this mutant termed type B the development of resistance to Oxytetracycline (30 μg/ml) was associated with a simultaneous loss of sensitivity to particular phages (HM 2 group). |
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