Conformation of Some Hexuronic Acids and d-Galactopyranosiduronic Acid Moiety in the Peracetylated and Permethylated Derivatives of Orange Pectic Acid in Solutions |
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| Authors: | Shigehiro Hirano Masatoshi Manabe Nobutaka Miyazaki Konoshin Onodera |
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| Institution: | Department of Agricultural Chemistry, Kyoto University, Kyoto, and Department of Chemistry, Belfer Graduate School of Science, Yeshiva University, New York, N.Y., 10033 |
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| Abstract: | The 1C conformation was estimated for α-d-galactopyranosiduronic acid moiety of pectic acid in the permethylated derivative dissolved in 1 n NaOD-D2O and in the peracetylated derivative dissolved in dimethyl sulfoxide-d6, and the C1 conformation was estimated for some derivatives of d-galactopyranuronic acid in chloroform-d by NMR spectroscopy. Random conformation of the whole macromolecule was estimated for pectic acid in water on the basis of no appearance of any induced Cotton effects in the 200 ~ 700 mμ region in the ORD spectra of pectic acid-anionic dye complexes. The conformation was supported by the fact that the rate of periodate oxidation of pectic acid at 5° was slightly decreased in comparison with that of amylase in 7 m urea solution.
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