Studies on the Protease of Pseudomonas

Proteinase productions of various species of the Pseudomonas genus were comparatively examined. Among them, Ps. aeruginosa was found to be the most suitable species for obtaining a proteinase in a high yield. The proteinase production of the organism was slight in natural medium, but remarkably high in shaking culture of synthetic medium containing glucose in a high concentration and a limited content of organic nitrogen. This medium found to be suitable for the species Ps. aeruginosa, also was remarkably similar with the medium suitable for Ps. myxogenes sp., which had been identified by us and used for this series of studies on the protease of Pseudomonas.