Distribution Patterns of 14C in the Labelled Vitamin B6 Prepared with the Cell-suspension of Achromobacter cycloclastes |
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| Authors: | Masahiko Ishida Hideo Nagayama Kensuke Shimura |
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| Institution: | Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University, Sendai |
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| Abstract: | The biosynthetic pathway of vitamin B6 (abbreviated as Be) has been studied with the cell-suspension of B6-producing bacteria, Achromobacter cycloclastes A.M.S. 6201. The distribution of 14C in the Be molecule prepared with the cell-suspensions containing glycerol-1,3-14C, glycerol-2-14C or γ-aminobutyric acid-U-14C was investigated by using three novel degradation methods. The results showed that carbon skeletons of glycerol and γ-aminobutyric acid were used for the formation of the major part of B6 carbon skeleton respectively. The implication of these compounds as precursors of B6 was discussed. |
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