Direct shoot organogenesis and plant regeneration in safflower |
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Authors: | A K A Mandal S Dutta Gupta |
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Institution: | (1) Floriculture Section, National Botanical Research Institute, 226 001 Lucknow, India;(2) Department of Agricultural and Food Engineering, Indian Institute of Technology, 721 302 Kharagpur, India |
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Abstract: | Summary Adventitious shoot buds were induced directly on the adaxial surface of the cotyledons of eight safflower cultivars after
14 d of culture initiation on Murashige and Skoog's (MS) medium supplemented with various levels of 6-benzylaminopurine (BA).
Maximum shoot organogenesis of 54.4% with 10.2 shoots per responding cotyledon was obtained with 8.87 μM BA in the cv. S-144. Regenerated shoots were classified into three groups on the basis of their morphological features and
were found to be correlated with the levels of BA. The highest number of normal shoots was obtained from 2.2 μM BA treatment. The regenerated shoots of group I (normal shoots) were rooted on half-strength MS medium supplemented with
5.3 μM α-naphthaleneacetic acid, 3% sucrose and 0.8% bacto-agar. Rooted plantlets were successfully transferred to soil and appeared
morphologically normal. Histological studies revealed that shoot buds originated adventitiously from subepidermal cells. |
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Keywords: | Carthamus tinctorius L histology in vitro shoot adventitious origin |
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