Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree |
| |
Authors: | P S Sha Valli Khan E Prakash K R Rao |
| |
Institution: | (1) Plant Tissue Culture, Laboratory, Tata Energy Research Institute, Darbari Seth Block, IHC, 110 003 New Delhi, India;(2) Plant Tissue Culture Laboratory, Department of Botany, Sri Venkateswara University, 517502 Tirupati, A.P., India |
| |
Abstract: | Summary A protocol has been developed for plantlet regeneration from seed callus of Bixa orellana L. Seeds demonstrated a high percentage of callus induction (63±7.3%) and a high yield (356±14.7 mg per seed) of white friable
callus on Murashige and Skoog (MS) medium containing 5.0 μM l-naphthaleneacetic acid (NAA) and 2.5μM N
6-benzyladenine (BA) within 6 wk of culture in the dark. Callus induction frequency was greater under 24h dark as compared
to 16h light/8h dark photoperiod or 24h light photoperiod. Increased myo-inositol (MI: 200mgl−1) and addition of ascorbic acid (AA: 200 mgl−1) to the culture medium positively improved callus induction frequency and growth. Shoot differentiation from white friable
seed callus was best using 10.0 μM BA and 5.0 μM NAA, where the highest percentage of calluses forming shools (74.9±4.8%), the highest number of shoots per callus (six or
seven) and the highest shoot-forming index (5.0) were obtained within 6 wk. Shoots elongated to 4 cm within 4 wk of transfer
onto MS medium devoid of growth regulators. Shoots were rooted using half-strength MS medium containing 5.0 μM indole-3-butyric acid (IBA). About 85% of these plants were established in pots containing pure garden soil and organic manure
after 3 wk of hardening. Regenerated plants were morphologically uniform with normal leaf, shape and growth patterns. These
plants are currently being screened for the presence of agronomically useful genetic variants. |
| |
Keywords: | annatto Bixa orellana Bixaceae plantlet regeneration seed callus |
本文献已被 SpringerLink 等数据库收录! |
|