炎症介质通过延迟嗜中性粒细胞凋亡促进内皮细胞的损伤

本研究采用分离纯化的人外周血NTP,以LPS/TNF激活后5:1和10:1与内皮细胞共同培养或隔离培养,加或不加10ng/ml IL-6、10%(v/v)烧伤血清,24h后观察NTP凋亡对体外培养内皮细胞损伤程度的影响及其关系。结果表明激活的NTP对内皮细胞没有明显损伤,但是加入IL-6和烧伤血清后,NTP凋亡延迟,内皮细胞受到损伤,表现为发生坏死,并且NTP对内皮细胞的损伤需要两种细胞的直接接触。

THE INFLAMMATORY MEDIATORS INDUCED ENDOTHELIAL CELL INJURY THROUGH DELAY NEUTROPHILS APOPTOSIS

This study aimed at elucidating the relationship between NTP apoptosis and endothelial cell injury. After isolation and purification, activated NTPs were incubated with endothelial cells directly or indirectly contact in a ratio of 5:1 and 10:1, meanwhile 10ng/ml IL-6 and 10% (v/v) burned serum added or not. Apoptosis and necrosis of endothelial cells were detected in situ. Activated NTP alone exerted no obviously detrimental effects on endothelial cells. However, when IL-6 or serum from bum patients was added, NTP apoptosis was delayed and endothelial cells were injured in the form of necrosis. Inflammatory mediators can delay NTP apoptosis, further research demonstrated that direct contact between the two types of cells was essential for NTP to impact injury on endothelial cells.