| 基于SSR标记的荔枝种质遗传多样性分析 |
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| 引用本文： | 曾 淇,李明芳,郑学勤. 基于SSR标记的荔枝种质遗传多样性分析[J]. 植物遗传资源学报, 2010, 11(3): 298-304 |
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| 作者姓名： | 曾 淇 李明芳 郑学勤 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海口,571101;海南大学农学院,海口,571101
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务项目 |
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| 摘 要： | 从53对本课题组自主开发设计的SSR特异引物对中筛选出22对多态性引物,对46份荔枝材料的基因组DNA进行扩增,共得到54个等位基因,其中每对引物的平均等位基因数为2.4,共获得23个特异性标记,各标记的平均观察杂合度值、平均期望杂合度和PIC值分别为0.451、0.355和0.507。其中L it6位点各数值最高,是最理想的选择标记。利用22对多态性引物对46份荔枝种质进行聚类分析,构建树状图,结果表明:大多数种质相似系数在0.63～0.95之间,说明它们之间的亲缘关系较近,并发现淮枝(广东)与淮枝(海南)两者可能为同名异物品种。
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| 关 键 词： | 荔枝 SSR标记 遗传多样性 |
Analysis of Genetic Diversity within Litchi Varieties based on SSR Markers |
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| ZENG Qi,LI Ming-fang,ZHENG Xue-qin. Analysis of Genetic Diversity within Litchi Varieties based on SSR Markers[J]. Journal of Plant Genetic Resources, 2010, 11(3): 298-304 |
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| Authors: | ZENG Qi LI Ming-fang ZHENG Xue-qin |
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| Affiliation: | ZENG Qi1,2,LI Ming-fang1,ZHENG Xue-qin1(1Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,2Hainan University of Agriculture,Haikou 571101) |
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| Abstract: | Twenty-two polymorphic primer pairs were selected from 53 SSR special primer pairs developed and designed by our laboratory to amplify genomic DNA of 46 litchi.23 locus-specific SSR markers were obtained,and 54 alleles were found and the average alleles per marker was 2.4.The average observed heterozygosity,expected heterozygosity and PIC of all markers were 0.451,0.355 and 0.507 respectively.Locus Lit6 had the highest number showed that it was an optimal marker.Then the selected polymorphic SSR markers wer... |
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| Keywords: | Litchi chinensis Sonn. SSR marker Genetic diversity |
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