金属硫蛋白在心肌细胞保护中与抗氧化酶的关系

目的：探讨金属硫蛋白（MT）在心肌细胞缺氧／复氧损伤（H／R）和预缺氧（HRC）保护中与抗氧化酶的关系。方法：建立培养的乳鼠心肌细胞缺氧／复氧模型,检测HPC、锌诱导、外源MT和MT抗体等处理下,心肌细胞MT含量变化及抗氧化酶（SOD,CAT,GSHpx）活力的相应变化。结果：HPC和锌诱导组MT含量及抗氧化酶的活力均显著高于H／R组P＜0. 05、P＜0.01）：外源MT处理后SOD活力显著高于对照组（P＜0.01）,CAT和GSHpx活力虽然显著低于对照组但显著高于H／R组（P＜0.01）;使用MT抗体后,酶活力最低。结论：MT参与HPC的心肌细胞保护作用,并通过保护抗氧化酶的活力的机制减轻H／R所致的心肌损伤。

Study on the relationship of metallothionein and antioxidases in protective mechanism of cardiomyocytes

Aim: To study the relationship of Metallothionein(MT) and antioxidases in hypoxia/reoxygenation(H/R) and hypoxic preconditioning(HPC). Methods: H/R and HPC model of rat cultured cardiomyocytes were established. Then the contents of MT and relevant changes of activities of antioxidases(SOD,CAT,GSHpx) of cardiomyocytes were determined while pretreated with the treatment: HPC. Zn 2 inducing MT or MT antibody. Results: In HPC and Zn 2 induced groups, all the contents of MT and the activities of antioxidases were obviously higher than those in H/R ( P <0.05, P <0.01 respectively). While using MT, the activities of SOD were progressively increased than control( P <0.05), CAT and GSHpx activities were lower than control( P <0.01), but were significantly higher than those in I/R group( P <0.01). In MT antibody groups, the activities of antioxidase were decreased extremely. Conclusion: MT has ettects on the cardiomyocytes protective role of HPC. And can afford more capacity for cardiomyocytes to tolerate the H/R damage by the mechanism of increasing the activities of antioxidase.