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| SSR分子标记开发策略及评价 | |
| 引用本文： | 张增翠,侯喜林. SSR分子标记开发策略及评价[J]. 遗传, 2004, 26(5): 763-768 |
| 作者姓名： | 张增翠 侯喜林 |
| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室，南京 210095National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095，China |
| 基金项目： | 国家高技术研究发展计划(863计划)，教育部高校骨干教师资助计划 |
| 摘 要： | SSR标记以其多态性丰富、提供遗传信息多、操作便利并在基因组中分散分布等优点已成为最受人们欢迎的分子标记之一，在许多领域广泛应用。但SSR标记的主要缺点是首先要从该物种中获取重复序列两侧的序列信息，并设计引物，而后才能被利用。综述了几种有代表性的SSR标记开发策略，旨在为各物种SSR标记的开发提供参考信息。Abstract: SSR molecular markers have been used widely in many genetic studies and become one of the most popular molecular markers due to their high polymorphism, abundant informativeness, convenience of assay by PCR and distribution throughout the genome. The major drawback of SSR molecular markers is that their primers need to be designed according to the isolated sequence flanking the SSR from species that are being examined for the first time. The aim of the present paper is to review the several representative methods of SSR isolation described in the literature and provide useful information in making appropriate choices among the large number of currently available options. |
| 关 键 词： | 策略Key words development SSR 开发 SSR |
| 文章编号： | 0253-9772(2004)05-0763-06 |
| 修稿时间： | 2003-06-20 |
Strategies for Development of SSR Molecular Markers |
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| ZHANG Zeng-Cui,HOU Xi-Lin njing Agricultural University,Nanjing ,China). Strategies for Development of SSR Molecular Markers[J]. Hereditas, 2004, 26(5): 763-768 | |
| Authors: | ZHANG Zeng-Cui HOU Xi-Lin njing Agricultural University Nanjing China) |
| Affiliation: | National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210 095, China. zengcui_zhang@hotmail.com |
| Abstract: | SSR molecular markers have been used widely in many genetic studies and become one of the most popular molecular markers due to their high polymorphism,abundant informativeness,convenience of assay by PCR and distribution throughout the genome.The major drawback of SSR molecular markers is that their primers need to be designed according to the isolated sequence flanking the SSR from species that are being examined for the first time.The aim of the present paper is to review the several representative methods of SSR isolation described in the literature and provide useful information in making appropriate choices among the large number of currently available options. |
| Keywords: | SSR |
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