Neuronal Control of Exocytosis and Calcium Homeostasis |
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Authors: | Fedirko N Vats Ju Klevets M Kruglikov I Voitenko N |
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Institution: | (1) Franko National University, L'viv, Ukraine;(2) National Academy of Sciences of Ukraine, Kyiv, Ukraine |
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Abstract: | We showed that 5 M acetylcholine (ACh) and 100 M norepinephrine (NE) cause increases in the total Ca2+ content in acinar cells by 30 and 87% and in the exocytosis intensity by 15 and 20%, respectively. Application of 5 M ACh and 100 M NE increased the free cytosolic Ca2+ concentration (Ca2+]
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) by 87 ± 2 and 140 ± 7 nM, respectively. Application of ACh and NE in a Ca2+-free external solution caused a Ca2+]
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increase that was 40 and 67% lower than in physiological solution. We postulate that the exocytosis developing upon neural stimulation of the gland results from generation of Ca2+ transients that are spreading from the basal to the apical region of the exocrine cell, where secretory granules are concentrated. |
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Keywords: | neurotransmitters exocytosis Ca2+ transients Ca2+ homeostasis acetylcholine norepinephrine |
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