Regulation of colony formation of differentiated chondrocytes in soft agar by transforming growth factor-beta |
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| Authors: | M Iwamoto K Sato K Nakashima H Fuchihata F Suzuki Y Kato |
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| Institution: | Department of Biochemistry, Faculty of Denistry, Osaka University, Japan. |
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| Abstract: | Fibroblast growth factor (FGF) induces colony formation by chondrocytes in soft agar (Y. Kato et al., J. Cell. Physiol., 1987), and the present study revealed that transforming growth factor-beta(TGF-beta) does not induce the same effect. TGF-beta did, however, increase the efficiency of colony formation by chondrocytes 3- to 4-fold in the presence of a maximal dose of FGF. Furthermore, TGF-beta decreased the concentrations of FGF needed for the induction of cell growth in soft agar by 40- to 100-fold. These results suggest that TGF-beta is involved in the control of cartilage growth possibly by increasing the responsiveness of chondrocytes to FGF. |
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