首页 | 本学科首页   官方微博 | 高级检索  
     


Isolation and Characterization of Lytic Properties of Bacteriophages Specific for M. haemolytica Strains
Authors:Renata Urban-Chmiel  Andrzej Wernicki  Diana St?gierska  Marta Dec  Anna Dudzic  Andrzej Puchalski
Affiliation:Sub-department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 20–033, Lublin, Poland, ; ContraFect Corporation, UNITED STATES,
Abstract:
ResultsEight bacteriophages were obtained, like typical of the families Myoviridae, Siphoviridae and Podoviridae. Most of the bacteriophages exhibited lytic properties against the M. haemolytica strains. Restriction analysis revealed similarities to the P2-like phage obtained from the strain M. haemolytica BAA-410. The most similar profiles were observed in the case of bacteriophages φA1 and φA5. All of the bacteriophages obtained were characterized by the presence of additional fragments in the restriction profiles with respect to the P2-like reference phage. In the analysis of PCR products for the P2-like reference phage phi-MhaA1-PHL101 ({"type":"entrez-nucleotide","attrs":{"text":"DQ426904","term_id":"90110542","term_text":"DQ426904"}}DQ426904) and the phages of the M. haemolytica serotypes, a 734-bp phage PCR product was obtained. The primers were programmed in Primer-Blast software using the structure of the sequence {"type":"entrez-nucleotide","attrs":{"text":"DQ426904","term_id":"90110542","term_text":"DQ426904"}}DQ426904 of reference phage PHL101.ConclusionsThe results obtained indicate the need for further research aimed at isolating and characterizing bacteriophages, including sequence analysis of selected fragments. Moreover, standardization of methods for obtaining them in order to eliminate M. haemolytica bacteria involved in the etiopathogenesis of BRDC is essential.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号