Synchrotron-based FTIR microspectroscopy of chili resistance induced by Bacillus subtilis strain D604 against anthracnose disease |
| |
| Authors: | Kanjana Thumanu Darawadee Wongchalee Mathukorn Sompong Piyaporn Phansak Toan Le Thanh Weravart Namanusart |
| |
| Affiliation: | 1. Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, Thailand;2. School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand;3. Bioactive Agro-Industry Co., Ltd, Seang-Sang, Nakhon Ratchasima, Thailand;4. Division of Biology, Faculty of Science, Nakhon Phanom University, Nakhon Phanom, Thailand;5. Crop Protection Department, College of Agriculture and Applied Biology, Can Tho University, Can Tho City, Vietnam;6. Department of Agricultural Technology and Environment, Faculty of Science and Liberal Arts, Rajamangala University of Technology, Nakhon Ratchasima, Thailand |
| |
| Abstract: | ![]()
The aim of this study was to determine the resistance mechanisms of chili induced by the Bacillus subtilis strain D604 using synchrotron FTIR microspectroscopy (SR-FTIR). In this study, the strain D604 reduced anthracnose disease severity in chili plants by approximately 31.10%. The SR-FTIR spectral changes from the epidermis and mesophyll leaf tissue revealed higher integral areas for the C=O ester from lipids, lignin, or pectin (1770–1700?cm?1) as well as polysaccharides (1200–900?cm?1) in the treated samples of D606 and distilled water and then challenge inoculation with chili anthracnose pathogen, Colletotrichum acutatum. The secondary structure of the Amide I protein failed to convert from alpha helices (centered at 1650?cm?1) to beta sheets (centered at 1600?cm?1) in the mesophyll of samples not treated with D604. This study suggested that the strain D604 induced resistance against anthracnose pathogen in chili by inducing cellular changes related to defense compounds involved in plant defense mechanism. |
| |
| Keywords: | SR-Fourier transform infrared chili anthracnose Bacillus subtilis biochemical composition hierarchical cluster analysis (HCA) induced resistance |
|
|
