Factors affecting pathogenicity of NPV preparations to the corn earworm,Heliothis armigera |
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Authors: | S J Tuan L C Tang R F Hou |
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Institution: | (1) Department of Entomology, National Chung Hsing University, 40227 Taichung, Taiwan, R.O.C.;(2) Present address: Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Wufeng, Taichung, Taiwan, Roc |
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Abstract: | Pathogenicity ofHeliothis nuclear polyhedrosis virus (HSNPV) to the corn earworm,Heliothis armigera, was studied using 3 different inoculative methods. The LD50 values of 4th-instar larvae inoculated with corn-fed, diet-fed and inoculum-imbiding method were 1.85×106, 2.55×105 and 1,22×103 PIBs/larva, respectively. The inoculum-imbiding is more sensitive and convenient for inoculatingH. armigera with HSNPV. The HSNPV product, Elcar®, was highly pathogenic toH. armigera, the LD50 values of 2nd-, 3rd- and 4th-instar larvae being 27, 83 and 1,221 PIBs/larva, respectively, as measured by the inoculum-imbiding method. The mortality of 4th-instar larvae caused by HSNPV was increased, but the incubation period was shortened with higher incubation temperatures. However, the high temperature at 35°C caused a lower mortality, and a prolongation of the median lethal time (LT50). Stability and persistence of HSNPV preparations were better in January–February and April–May than in June–July and October–November periods when sprayed on corn silks under field conditions. The HSNPV was inactivated by weak alkaline dew (pH 8.1) collected from soybean leaves, but it remained active on those from corn, tomato and asparagus with pH 7.2–7.3. The artificial heavy rainfall of 242 mm/h for 30 min did not wash off HSNPV preparations sprayed on the corn silks. |
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Keywords: | Heliothis armigera corn earworm nuclear polyhedrosis virus Elcar? |
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