| 不同有机酸对粘质沙雷氏菌合成2,3-丁二醇的影响 |
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| 引用本文： | 张燎原,黄晓燕,孙建安,魏东芝,沈亚领.不同有机酸对粘质沙雷氏菌合成2,3-丁二醇的影响[J].工业微生物,2012,42(4):1-6. |
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| 作者姓名： | 张燎原 黄晓燕 孙建安 魏东芝 沈亚领 |
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| 作者单位： | 张燎原 (福建农林大学,生物农药与化学生物学教育部重点实验室,福州350002) ； 黄晓燕 (福建农林大学,生物农药与化学生物学教育部重点实验室,福州350002) ； 孙建安 (华东理工大学,生物反应器国家重点实验室,鲁华生物技术研究所,上海200237) ； 魏东芝 (华东理工大学,生物反应器国家重点实验室,鲁华生物技术研究所,上海200237) ； 沈亚领 (华东理工大学,生物反应器国家重点实验室,鲁华生物技术研究所,上海200237) ； |
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| 基金项目： | 福建省自然科学基金项目,福建省教育厅A类科技项目 |
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| 摘 要： | 考察了不同的短链有机酸对粘质沙雷氏菌合成2，3－丁二醇的影响，结果表明乙酸、乳酸、丙酮酸、琥珀酸、延胡索酸和柠檬酸均能在一定程度上提高2，3－丁二醇的产量，其中乙酸的效果最为明显，在基础培养基中添加6g／L乙酸，与对照相比，2，3．丁二醇的产量提高了91．06％，此外菌体干重也提高了58．28％。为了揭示其中的调控机制，构建了启动子：lacZ融合报告载体，fncz活性测定显示六种有机酸均可提高报告基因B．半乳糖苷酶的表达，其中乙酸可提高B．半乳糖苷酶活性近4倍，暗示六种有机酸促进2，3－丁二醇的合成可能与诱导该合成途径相关基因的表达有关。
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| 关 键 词： | 粘质沙雷氏菌 2 3-丁二醇 有机酸 融合表达分析 |
Effects of different organic acids on 2,3-butanediol production by Serratia marcescens |
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| ZHANG Liao-yuan,HUANG Xiao-yan,SUN Jlan-an,WEI Dong-zhi,SHEN Ya-ling.Effects of different organic acids on 2,3-butanediol production by Serratia marcescens[J].Industrial Microbiology,2012,42(4):1-6. |
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| Authors: | ZHANG Liao-yuan HUANG Xiao-yan SUN Jlan-an WEI Dong-zhi SHEN Ya-ling |
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| Institution: | 1. Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education, Fuzhou 350002 ; 2. East China University of Science ＆ Technology, State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, Shanghai, 200237 ) |
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| Abstract: | Effects of different short chain organic acids on 2, 3-butanediol production by Serratia marcescens were investigated. Results showed that acetate, lactate, pyruvate, succinate, fumarate and citrate could improve the yield of 2,3- butanediol. Among these organic acids, acetate of 6 g/L provided the increase of 91.06% for 2,3-butanediol yield and 58.28% for cell dry weight compared with the control. To elucidate the regulatory mechanism, a report plasmid with the promoter from 2,3-butanediol pathway and a lacZ gene was constructed. ~-galactosidase activities assay indicated that six organic aicds could improve the expression of lacZ gene. Approximately 4-fold β-galactosidase activities increase was observed in the presence of acetate under the control. These results showed six organic acids promoted 2,3-butanediol biosynthesis through inducing the expression of these genes involved 2,3-butanediol biosynthetic pathway in Serratia |
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| Keywords: | Serratia marcescens 2 3-butanediol organic acids fusion expression |
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