Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture |
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Authors: | TA Zecchini RJ Smith |
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Institution: | (1) Cantab Pharmaceuticals Research Ltd., 310 Cambridge Science Park, Cambridge, CB4 0WG, UK |
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Abstract: | Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk
material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1
at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactors. The cells are infected at an MOI of 0.01 pfu cell-1 and
the culture harvested 60–72 h later. The infected cells are removed from the microcarriers by the addition of a hypotonic
saline and the virus released by low-pressure disruption techniques. Virus titres achieved are compared to the standard roller
bottle process. The resulting material is the starting point for the purification of the DISC-HSV virus.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | CR2 cells Cytodex-1 DISC-HSV hightitre virus Vero |
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