Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1,2-epoxyoctane |
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| Authors: | J Maritz HM Krieg CA Yeates AL Botes JC Breytenbach |
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| Institution: | (1) Departments of Pharmaceutical Chemistry, Potchefstroom University for CHE, Private Bag X6001, 2520 Potchefstroom, South Africa;(2) Chemistry and Biochemistry, Potchefstroom University for CHE, Private Bag X6001, 2520 Potchefstroom, South Africa;(3) CSIR Bio/Chemtek, Private Bag X2, 1645 Modderfontein, South Africa |
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| Abstract: | Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilised biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M were utilised for bead preparation. |
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| Keywords: | calcium alginate epoxide hydrolase immobilisation Rhodosporidium toruloides (R S)-1 2-epoxyoctane |
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