| 盐芥miR396的表达分析、前体克隆及靶基因预测 |
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| 引用本文： | 宫晓琳,熊雪梅,吴莹,王洋.盐芥miR396的表达分析、前体克隆及靶基因预测[J].植物生理学通讯,2014(11):1692-1698. |
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| 作者姓名： | 宫晓琳 熊雪梅 吴莹 王洋 |
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| 作者单位： | 东北林业大学盐碱地生物资源环境研究中心东北油田盐碱植被恢复与重建教育部重点实验室,哈尔滨150040 |
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| 基金项目： | 国家自然科学基金（31170368） |
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| 摘 要： | 利用实时定量PCR的方法分析了tsa-mi R396在盐芥不同组织的表达情况及盐、冷胁迫下的表达量变化,结果显示tsami R396在盐芥的不同组织均有表达,且在盐芥幼苗受盐、冷胁迫处理2 h后其表达量明显上升,随后呈波动性变化。利用在线软件ps RNATarget预测到25个tsa-mi R396的靶基因并进行功能分类。成功克隆tsa-mi R396前体,并利用Mfold在线软件分析该前体能够折叠形成茎环结构。
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| 关 键 词： | 盐芥 miRNA 实时定量PCR 靶基因 前体 |
Expression Analysis,Precursor Cloning and Target Prediction of mi R396 in Thellungiella salsuginea |
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| GONG Xiao-Lin,XIONG Xue-Mei,WU Ying,WANG Yang.Expression Analysis,Precursor Cloning and Target Prediction of mi R396 in Thellungiella salsuginea[J].Plant Physiology Communications,2014(11):1692-1698. |
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| Authors: | GONG Xiao-Lin XIONG Xue-Mei WU Ying WANG Yang |
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| Institution: | (Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Alkali Soil Natural Environ- mental Science Center, Northeast Forestry University, Harbin 150040, China) |
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| Abstract: | In this study, we analyzed the expression levels of tsa-mi R396 in different tissues and response to the salt and cold stresses by real-time quantitative RT-PCR. The results showed that tsa-mi R396 widely expressed in different tissues of Thellungiella salsuginea and its expression level was strongly up-regulated at 2 hours of salt or cold treatment and then changed in volatility. Using the ps RNATarget online 25 target genes of tsami R396 were predicted and classifi cated according to their functions. Moreover, the precursor of tsa-mi R396 was successfully cloned and could fold into the stem-loop structure by Mfold web server. |
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| Keywords: | Thellungiella salsuginea miRNA real-time quantitative PCR target gene precursor |
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