| 红豆杉细胞悬浮培养中不同添加物对细胞生长和紫杉醇合成的影响 |
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| 引用本文： | 李干雄,黄巧明.红豆杉细胞悬浮培养中不同添加物对细胞生长和紫杉醇合成的影响[J].植物资源与环境学报,2000,9(3):11-14. |
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| 作者姓名： | 李干雄 黄巧明 |
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| 作者单位： | 广东梅县梅雁生物工程研究所,梅县,514787 |
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| 基金项目： | 梅雁经济发展总公司资助 |
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| 摘 要： | 采用正交实验检测红豆杉（Ｔａｘｕｓ ｃｈｉｎｅｎｓｉｓ（Ｐｉｌｇｅｒ）Ｒｅｈｄ．）细胞悬浮培养中水杨酸、Ｄ－果糖、甘露醇和硫酸镧对细胞生长和紫杉醇（ｔａｘｏｌ）积累的影响。添加１０ｇ／ＬＤ－果糖，可使细胞的鲜重和干重明显增加；添加６０ｇ／Ｌ甘露醇使细胞的鲜重和干重明显减少；１ｍｇ／Ｌ水杨酸仅使细胞鲜重增加，对干重影响不明显；硫酸镧对细胞生长无明显影响。单独添加这４种物质，紫杉醇含量均下降，同时添加
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| 关 键 词： | 紫杉醇 红豆杉 悬浮培养 正交设计 |
| 文章编号： | 1004-0978(2000)03-0011-04 |
| 修稿时间： | 2000年4月5日 |
Effects of different additions on cell growth and taxol biosynthesis in cell suspension culture of Taxus chinensis (Pilger) Rehd |
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| LI Gan-xiong,HUANG Qiao-ming.Effects of different additions on cell growth and taxol biosynthesis in cell suspension culture of Taxus chinensis (Pilger) Rehd[J].Journal of Plant Resources and Environment,2000,9(3):11-14. |
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| Authors: | LI Gan-xiong HUANG Qiao-ming |
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| Abstract: | The effects of salicylic acid, D\|fructose, mannitol and lanthanum sulfate on cell growth and taxol biosynthesis in cell suspension culture of \%Taxus chinensis \%(Pilger) Rehd. were studied by using orthogonal design experiment. The increase or decrease of fresh and dry weight of cell occured when 10 g/L D\|fructose or 60 g/L mannitol was added respectively; only fresh weight could be increased by adding 1 mg/L salicylic acid while no obvious effect was on cell growth by lanthanum sulfate. Taxol amount decreased when each chemical was added to culture separately but it could be improved 127% in contrast to control if D\|fructose and lanthanum sulfate were added simultaneously. |
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| Keywords: | taxol \%Taxus chinensis \%(Pilger) Rehd cell suspension culture orthogonal design |
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