A quick and simple biostrip technique for detection of lactose |
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Authors: | Sandeep K. Sharma Neeta Sehgal Ashok Kumar |
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Affiliation: | (1) Centre for Biochemical Technology, Delhi University Campus, Mall Road, Delhi, 110007, India;(2) Department of Zoology, Delhi University, Delhi, 110007, India |
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Abstract: | A quick, simple and economical biostrip technology was developed for estimation of lactose by immobilizing -galactosidase, galactose oxidase and horseradish peroxidase on to a polymeric support. The biostrip is dipped in milk or milk products and, from the colour that develops from an added chromogen, the concentration of lactose can be estimated from < 20 to 100+g l–1. The biostrips may be used in dairy industries, hospitals and remote areas where expensive instruments are not available. |
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Keywords: | /content/k6450530hh2u6472/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-galactosidase biostrip lactase lactose lactose intolerance |
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