Comparison of RAPD and RFLP markers for mapping F2 generations in maize (Zea mays L.) |
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Authors: | V H Beaumont J Mantet T R Rocheford J M Widholm |
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Institution: | (1) Südwestdeutsche Saatzucht, Im Rheinfeld 1-13, 76437 Rastatt, Germany;(2) Semences Cargill/Ets Lesgourgues, Boissay BP17, 28310 Toury, France;(3) Department of Crop Sciences, University of Illinois at Urbana-Champaign, 1201 W. Gregory, 61801 Urbana, IL, USA |
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Abstract: | The F2 generations from two maize crosses were used to compare the ability of RAPD and RFLP marker systems to create a genetic linkage map. Both RFLPs and RAPDs were shown to provide Mendelian-type markers. Most of the RFLPs (80%) could be placed with a good level of certainty (LOD>4) on the genetic linkage map. However, because of their dominant nature, only between 37% and 59% of the RAPDs could be placed with such a LOD score. The use of combined data from RFLPs and RAPDs increases the level of information provided by RAPDs and allows the creation of a combined RFLP/RAPD genetic linkage map. Thus, the RAPD technique was found to be a powerful method to provide improved probes coverage on a previously created RFLP map and to locate markers linked to chromosomal regions of interest. |
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Keywords: | Molecular markers RFLP RAPD Genetic linkage map |
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