Plantlet regeneration from callus initiated from flower buds in the wild species Allium senescens var. minor |
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Authors: | Ashalatha S Nair Bong Bo Seo |
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Institution: | (1) Present address: Department of Biology, Kyungpook National University, 702-701 Taegu, South Korea;(2) Department of Botany, University of Kerala, Karyavattom, 695 581 Trivandrum, India |
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Abstract: | Callus regeneration was observed from flower buds of Allium senescens var. minor inoculated in BDS, MS or B5 medium supplemented with 4.4 M benzyladenine alone or in combination with 4.5 M 2,4-dichlorophenoxy-acetic acid (2,4-d), with 2,4-d and kinetin (4.5 M/4.6 M) or with 5.3 M naphthaleneacetic acid. Ovules enlarged initially but the embryogenic tissue degenerated as callus development progressed from the nectar regions of the petals. Shoot buds and leaf primordia developed from the meristematic protuberances that originated from the surface of the callus. BDS medium with 4.5 M 2,4-d and 13.3 M BA was most suitable for shoot multiplication. The regenerated shoots were rooted in respective liquid medium without any growth regulators and successfully transferred to soil with 90% survival rate.Abbreviations BA
N6-benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- NAA
-naphthaleneacetic acid |
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Keywords: | in vitro culture organogenesis shoot buds |
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