Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds |
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| Authors: | S Kostia J Kantanen M Kolkkala & S-L Varvio |
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| Institution: | Department of Biosciences, Division of Genetics, PO Box 56 (Viikinkaari 5), FIN-00014 University of Helsinki, Finland Animal Breeding Section, Agricultural Research Centre, 31600 Jokioinen, Finland |
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| Abstract: | The applicability of single strand conformation polymorphism (SSCP) analysis for major histocompatibility complex (MHC) genotyping in sheep was studied. A panel of Ovar-DRB1 exon 2 'allele fingerprints' was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing sequences are used as references in SSCP analysis. In this study, seven new exon 2 sequences and 19 different alleles in total were detected from 31 animals of Finnish and Russian sheep breeds. Ovar-DRB1 * 0201 was detected in all the six grey Finnsheep animals included in this study, suggesting reduced MHC diversity within these animals. |
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| Keywords: | allele fingerprinting genotyping MhcOvar SSCP |
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