Biochemical studies of H-2K antigens from a group of related mutants. I. Identification of a shared mutation in B6-H-2
bm5
and B6-H-2
bm16 |
| |
Authors: | Karen M Yamaga Gertrude M Pfaffenbach Larry R Pease Diane Mc Governs Tosiki Nisizawa Roger W Melvold Henry I Kohn Dr Stanley G Nathenson |
| |
Institution: | (1) Department of Microbiology and Immunology and Department of Cell Biology, Albert Einstein College of Medicine, 10461 Bronx, New York;(2) Shields Warren Radiation Laboratory, Harvard Medical School, Boston, Massachusetts;(3) Present address: Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Honolulu, Hawaii;(4) Present address: National Institute of Health, Tokyo, Japan;(5) Present address: Section of Medical Oncology, Department of Medicine and Microbiology-Immunology, Northwestern University Medical School, 60601 Chicago, Illinois;(6) Department of Microbiology and Immunology, Albert Einstein College of Medicine, 10461 Bronx, New York |
| |
Abstract: | Structural studies of the H-2 gene products from a group of five closely related but independent C57BL/6 H-2 mutant mice were undertaken. Each of the mutants exhibits reciprocal graft rejection with the parent. The group is remarkable, however, because each member of this group can accept skin grafts from any other member. The results of biochemical analysis of the H-2 glycoproteins from two of these related mutants, bm5 and bm16, are presented in this report. Evidence is given that the H-2K molecules from these two mutants are identical to each other based on comparative tryptic peptide mapping profiles with the parent. From partial amino acid sequence analysis, K products of both mutants have at least one common difference from the parental type located at residue number 116. Definitive studies established that in both bm5 and bm16 a tryosine found in the parent molecule is substituted with a phenylalanine in the mutant. These results show that a biochemical difference between the K products of the two mutants and of the parent can be detected, that the mutants appear to be identical with one another even though they arose independently, and that they differ from the other H-2K
b mutants analyzed.Abbreviations used in this paper B6
C57BL/6Kh
-
bm5
B6-H-2bm5
-
bm6
B6-H-2
bm6
-
bm7
B6.C-H-2
bm7
-
bm9
B6.C-H-2
bm9
-
bm16
B6-H-2
bm16
- D
H-2D
- K
H-2K
- MHC
major histocompatibility complex |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|