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Transfer and expression of the cryIVB and cryIVD genes of Bacillus thuringiensis subsp. israelensis in Bacillus sphaericus 2297 |
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| Authors: | Sandrine Poncet Armelle Delecluse Guido Anello ré Klier Georges Rapoport |
| Institution: | Unitéde Biochimie Microbienne, Institut Pasteur, URA 1300 Centre National de la Recherche Scientifique, 25 rue du Docteur Roux, 75724 Paris, Cedex 15, France; Unitédes Bactéries Entomopathogènes, Institut Pasteur, 25, rue du Docteur Roux, 75724, Paris Cedex 15, France; Universitàdegli Studi di Ferrara, Istituto di Biochimica, via Borsari 46, 44100 Ferrara, Italy |
| Abstract: | Abstract The genes encoding the CryIVB and CryIVD crystal polypeptides of B. thuringiensis subsp. israelensis were cloned indepently on a stable shuttle vector, and transfered into B. sphaericus 2297. Recombinant cells expressed the B. thuringiensis toxins during sporulation and were shown to be toxic to Aedes aegypti fourth instar larvae, whereas the parental strain was not. |
| Keywords: | Bacillus sphaericus Bacillus thuringiensis subsp israelensis toxins Larvicidal activity Gene transfer Gene expression |