Compensatory increase in<Emphasis Type="Italic"> ahpC</Emphasis> gene expression and its role in protecting<Emphasis Type="Italic"> Burkholderia pseudomallei</Emphasis> against reactive nitrogen intermediates |
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Authors: | Email author" target="_blank">Suvit?LoprasertEmail author Ratiboot?Sallabhan Wirongrong?Whangsuk Skorn?Mongkolsuk |
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Institution: | (1) Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210, Thailand;(2) Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand |
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Abstract: | In the human pathogen Burkholderia pseudomallei, katG encodes the antioxidant defense enzyme catalase-peroxidase. Interestingly, a B. pseudomallei mutant, disrupted in katG, is hyperresistant to organic hydroperoxide. This hyperresistance is due to the compensatory expression of the alkyl hydroperoxide reductase gene (ahpC) and depends on a global regulator OxyR. The KatG-deficient mutant is also highly resistant to reactive nitrogen intermediates (RNI). When overproduced, the B. pseudomallei AhpC protein, protected cells against killing by RNI. The levels of resistance to both organic peroxide and RNI returned to those of the wild-type when the katG mutant was complemented with katG. These studies establish the partially overlapping defensive activities of KatG and AhpC. |
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Keywords: | Melioidosis Alkyl hydroperoxide reductase Catalase-peroxidase |
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