牛猪轮状病毒血清分型及其抗原性比较

SEROTYPING AND ANTIGENIC COMPARISON OF SOME ANIMAL ROTAVIRUSES ISOLATED IN CHINA

Eight strains of rotavirus isolated from diarrheal animals ( 4 from calves and 4 from piglets ) in China were examined for serotyping and antigenic relationship with reference animal rotavirus strains such as bovine NCDV, porcine OSU, simian SA-11 and human rotavirus Wa strain. Two-way cross neutralization tests showed no antigenic difference between all 4 local strains of bovine rotavirus ( HN-7, BRV007, BRV 014 and BRV6555)and reference NCDV, so they belonged to rotavirus serotype 6 (NCDV serotype or bovine rotavirus serotype 1). Meanwhile, 4 strains of Chinese porcine rotavirus could be differentiated into 2 se rotypeSs Li99 was neutralised to a high liter with antiserum against re ference OSU virus and considered related to OSU ( serotype 5 or porcine serotype 1), the other three strains( Lin7l, Nan86 and Jiangl50) was highly similar to each other but showed obvious difference to Li99 and did not react with antiserum against OSU.They were considered tentatively as porcine rotavirus serotype 2. All the strains of bovine and porcine rotavirus did not cross neutralise with simian SA-11 and human Wa strain. There was also no antigenic relationship between bovine rota viruses and porcine rotaviruses.