Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera |
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Authors: | Laurie Burnett Stephen Yarrow Bin Huang |
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Institution: | (1) Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1 Mississauga, Ont., Canada |
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Abstract: | Summary Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspore embryogenesis, buds (2–3mm in length, containing mid-late uninucieate microspores) were collected from older plants (2 months old) and microspores isolated and washed in iron-free B5 medium. NLN medium with its iron content reduced to half was beneficial for initial microspore culture. An elevated temperature(33–35°C) during the first day of culture, followed by maintenance at 25°C resulted in dozens of embryos from each isolation (about 100 buds). Seeds were obtained from plants regenerated from microsporederived embryos after colchicine treatment. |
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Keywords: | Microspore embryogenesis B rapa genotypes iron toxicity |
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