| 腺病毒载体介导CEA基因元件调控自杀基因治疗 |
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| 引用本文： | 许德华,戈凯,蒋琼,郑仲承,刘新垣.腺病毒载体介导CEA基因元件调控自杀基因治疗[J].生物化学与生物物理进展,1998,25(5):444-448. |
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| 作者姓名： | 许德华 戈凯 蒋琼 郑仲承 刘新垣 |
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| 作者单位： | 中国科学院上海生物化学研究所, 上海 200031;中国科学院上海生物化学研究所, 上海 200031;中国科学院上海生物化学研究所, 上海 200031;中国科学院上海生物化学研究所, 上海 200031;中国科学院上海生物化学研究所, 上海 200031 |
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| 摘 要： | 构建以CEA启动子控制HSV-TK基因表达的复制缺陷型腺病毒载体(AdCEATK).纯化的重组腺病毒滴度达1×1012pfu/ml.CEA阴性的HeLa细胞感染AdCMVTK后对丙氧鸟苷(GCV)很敏感，而感染了AdCEATK后不被GCV杀伤.与此相反CEA阳性的LoVo细胞中AdCMVTK和AdCEATK都有很好的表达活性，说明CEA启动子有良好的细胞专一性.AdCEATK/GCV系统还有明显的旁杀伤效应.此载体将有助于实现对CEA阳性肿瘤的专一性自杀基因治疗.
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| 关 键 词： | 腺病毒载体， 癌胚抗原基因启动子，单纯疱疹病毒胸苷激酶(HSV-TK)基因，基因治疗 |
| 收稿时间： | 1997/7/17 0:00:00 |
| 修稿时间： | 1997/11/20 0:00:00 |
CEA Gene Element Control Suicide Gene Therapy Mediated by the Adenovirus Vector |
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| XU De-hu,GE Kai,JIANG Qiong,ZHENG Zhong-cheng and LIU Xin-yuan.CEA Gene Element Control Suicide Gene Therapy Mediated by the Adenovirus Vector[J].Progress In Biochemistry and Biophysics,1998,25(5):444-448. |
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| Authors: | XU De-hu GE Kai JIANG Qiong ZHENG Zhong-cheng and LIU Xin-yuan |
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| Institution: | Shanghai Institute of Biochemistry,the Chinese Academy of Sciences,Shanghai 200031,China;Shanghai Institute of Biochemistry,the Chinese Academy of Sciences,Shanghai 200031,China;Shanghai Institute of Biochemistry,the Chinese Academy of Sciences,Shanghai 200031,China;Shanghai Institute of Biochemistry,the Chinese Academy of Sciences,Shanghai 200031,China;Shanghai Institute of Biochemistry,the Chinese Academy of Sciences,Shanghai 200031,China |
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| Abstract: | A replication-defective recombinant adenovirus vector containing HSV-TK gene under the control of CEA promoter was constructed (AdCEATK). Titer of the purified recombinant adenovirus is about 1012pfu/ml. HeLa cell (CEA-negative cell) infected with AdCMVTK became sensitive to GCV, while HeLa cell infected with AdCEATK was not. On the contrary, the LoVo cell infected with both AdCMVTK and AdCEATK were sensitive to GCV. It was shown that CEA promoter has a good tumor-specificity. Significant bystander effect was observed in the AdCEATK/GCV system too. This system should be useful for tumor-specificity suicide gene therapy of CEA-positive tumors. |
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| Keywords: | adenovirus vector CEA promoter herpes simplex virus thymidine kinase (HSV-TK) gene gene therapy |
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