Modification of glycerolipid metabolism in L-M fibroblasts by an unnatural amino-alcohol, N-isopropylethanolamine. |
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Authors: | M L Blank C Piantadosi K S Ishaq F Snyder |
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Institution: | 1. Medical Division, Oak Ridge Associated Universities, Oak Ridge, TN 37830 U.S.A.;2. Department of Medicinal Chemistry, School of Pharmacy, University of North Carolina, Chapel Hill, NC 27514 U.S.A. |
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Abstract: | The unnatural amino-alcohol, N-isopropylethanolamine, is incorporated into a phospholipid by monolayers of L-M fibroblasts. This phospholipid was identified as 1,2-diacyl--glycero-3-phosphoisopropylethanolamine by using chemical and enzymatic procedures combined with thin-layer and gas-liquid chromatography. Since the phospho-N-isopropylethanolamine moiety is removed by phospholipase C, the stereochemistry of the phospholipid analog is identical to naturally occurring phosphoglycerides. Incubation of cells in 10 mM N-isopropylethanolamine inhibited the incorporation of 14C]choline and 14C]ethanolamine into phospholipids and stimulated the incorporation of 1-14C]palmitic acid and 1-14C]hexadecanol into triacylglycerols and alkyldiacylglycerols. These results indicate that N-isopropylethanolamine affects glycerolipid synthesis at the diradylglycerol branch point. |
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Keywords: | Submit requests for reprints to Dr Snyder |
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