Nonsense-suppressor mutants of Erwinia carotovora subsp. carotovora |
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Authors: | Jay CD Hinton Michel CM Pérombelon George PC Salmond |
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Institution: | Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K. |
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Abstract: | Abstract A promiscuous plasmid (pLM2) carrying amber mutations in two antibiotic-resistance genes was transferred to a derivative of Erwinia carotovora subsp. carotovora strain SCRI193. Following mutagenesis, two putative amber-suppressing mutants of this strain were isolated. The genotype of these mutants was confirmed by use of rep am plasmid-specific phage. This constitutes the first isolation of amber-suppressing mutants in Erwinia spp. |
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Keywords: | Erwinia pLM2 mutagenesis |
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