Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis |
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| Authors: | B E Froman R C Tait C I Kado R L Rodriguez |
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| Institution: | 1. Department of Genetics, University of California, Davis, CA 95616 U.S.A. Tel. (916)752-0325;1. Department of Plant Pathology, University of California, Davis, CA 95616 U.S.A. Tel. (916)752-0325 |
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| Abstract: | A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C decreases CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA. |
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| Keywords: | Recombinant DNA enzyme purification blunt and cohesive termini phage λ bp base pairs kb 1000 bp XB extraction buffer (see MATERIALS AND METHODS section b) |
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