| 促红细胞生成素对神经干细胞缺氧性损伤保护作用的实验研究 |
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| 引用本文： | 赵舒武,高英茂,汪涛,王晓玲,张静.促红细胞生成素对神经干细胞缺氧性损伤保护作用的实验研究[J].中国组织化学与细胞化学杂志,2008,17(3). |
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| 作者姓名： | 赵舒武 高英茂 汪涛 王晓玲 张静 |
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| 作者单位： | 1. 天津中医药大学基础学院组织胚胎学教研室,天津,300193
2. 山东大学医学院组织胚胎学教研室,济南,250012 |
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| 摘 要： | 目的观察外源性EPO对神经干细胞缺氧性损伤的保护作用,为缺氧缺血性脑损伤的治疗提供新思路。方法从孕11.5d（E11.5d）大鼠获得神经干细胞,经无血清培养基悬浮培养并传代,对所获细胞的自我增殖、自我更新及其多分化潜能进行检测。取传3代神经干细胞中添加不同剂量的EPO,在5%O2培养箱中培养120h。通过计数干细胞克隆形成率和MTT法检测神经干细胞的增殖情况。于含血清分化培养基中加入不同剂量的EPO,用NSE和GFAP免疫细胞化学染色观察神经干细胞的分化情况。采用AnnexinⅤ-FITC/PI染色,激光扫描共聚焦显微镜观察、检测细胞凋亡率。结果加入EPO后神经干细胞的克隆形成率和MTT检测的OD值显著增高,细胞凋亡率显著下降,NSE阳性细胞的比例明显升高,其作用随剂量增加而增大,50U/ml时作用最大。结论EPO对神经干细胞缺氧性损伤具有明显的保护作用,并可促进神经干细胞向神经元方向分化。EPO的这种作用随剂量增加而增大,50U/ml时达高峰。
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| 关 键 词： | 促红细胞生成素 神经干细胞 缺氧 |
PROTECTIVE EFFECT OF EPO ON HYPOXIA-IMPAIRED NSCs |
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| Zhao Shuwu,Gao Yingmao,Wang Tao,Wang Xiaoling,Zhang Jing.PROTECTIVE EFFECT OF EPO ON HYPOXIA-IMPAIRED NSCs[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(3). |
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| Authors: | Zhao Shuwu Gao Yingmao Wang Tao Wang Xiaoling Zhang Jing |
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| Institution: | Zhao Shuwu1,Gao Yingmao2,Wang Tao1,Wang Xiaoling1,Zhang Jing2 (1Department of Histology , Embryology,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,2Department of Histology , Embryology,School of Medicine,Sh,ong University,Jinan 250012,China) |
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| Abstract: | Objective To observe the protection of exogenous EPO on NSCs impaired by hypoxia and to provide a new therapeutic method for hypoxic-ischemic brain injury.Methods The NSCs of E11.5d were isolated,cultured and passaged.Different concentrations of EPO were added to cultures during proliferation of NSCs exposed to 5%O2 for 120h.Proliferation of NSCs was evaluated by MTT method and calculating clones.During the differentiation of NSCs with 10% FBS,different concentrations of EPO were added.The ratio of the diff... |
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| Keywords: | Erythropoietin(EPO) Neural stem cell(NSC) Hypoxia |
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