High frequency stable transformation ofArabidopsis thaliana by particle bombardment |
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| Authors: | Misa Takahashi Hiromichi Morikawa |
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| Institution: | (1) Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Kagamiyama, 739 Higashi-Hiroshima, Japan |
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| Abstract: | Root explants ofArabidopsis thaliana ecotype C24 were bombarded with the plasmid pCH harboring the hygromycin phosphotransferase gene (hpt). A selection condition with post-bombardment culture of 3 days followed by culture with 20 mgl−1 hygromycin gave the highest yield of transformants. More than 44% of explant clumps formed transformant shoots. |
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| Keywords: | Arabidopsis thaliana Hygromycin phosphotransferase gene (hpt) Particle bombardment Transgenic plant |
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