| 转金属硫蛋白基因(MT_1)烟草耐NaCl胁迫能力 |
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| 引用本文： | 周博如,王雷,吴丽丽,姜廷波.转金属硫蛋白基因(MT_1)烟草耐NaCl胁迫能力[J].生态学报,2010,30(15):4103-4108. |
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| 作者姓名： | 周博如 王雷 吴丽丽 姜廷波 |
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| 作者单位： | 东北林业大学,林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 |
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| 基金项目： | 黑龙江省重大科技攻关资助项目(GA09B201-4); 国家重点基础研究发展计划资助项目（2009CB125906） |
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| 摘 要： | 为明确柽柳(Tamarix sp.)金属硫蛋白(MT1)基因过量表达对提高植物耐NaCl能力的作用,对转MT1因烟草进行分子检测和生理特性分析,结果表明具有卡那霉素抗性的转基因植株经RT-PCR Southern杂交均表现为阳性,说明外源MT1基因已整合到烟草基因组,并且得到了表达。金属硫蛋白基因的过量表达提高了转基因烟草植株的耐NaCl能力,表现为在含有150mmol/L和300mmol/L NaCl的MS培养基上,转基因植株的株高和鲜重均明显优于非转基因株系;在生理性状上表现为转基因植株丙二醛(MDA)含量明显低于非转基因株系,而超氧化物歧化酶(SOD)、过氧化物酶(POD)活性比非转基因株系明显增加。
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| 关 键 词： | 烟草 柽柳 金属硫蛋白 耐盐 转基因 |
| 收稿时间： | 2009/10/9 0:00:00 |
| 修稿时间： | 2010/3/22 0:00:00 |
Characterization of transgenic tobacco overexpressing metallothionein gene
(MT1) on NaCl stress |
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| zhouboru.Characterization of transgenic tobacco overexpressing metallothionein gene
(MT1) on NaCl stress[J].Acta Ecologica Sinica,2010,30(15):4103-4108. |
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| Authors: | zhouboru |
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| Institution: | ZHOU Boru,WANG Lei,WU Lili,JIANG Tingbo Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | Saline-alkali stress is one of the important environmental factors that affect plant growth and development.Due to the development of irrigated agriculture and fertilizer misuse,soil salinization and secondary salinization have become main constraints for sustainable development of agricultural production.Methallothionein genes,which can be induced by environmental stresses such as saline-alkali,are resilience-related genes.Methallothionein genes are the most abundantly expressed in Tamarix sp.under the NaH... |
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| Keywords: | tobacco Tamarix sp methallothionein salt-tolerance genetic transformation |
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