X-ray interferometry of the axial movement of myosin heads during muscle force generation initiated by T-Jump |
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Authors: | N A Koubassova S Y Bershitsky M A Ferenczi P Panine T Narayanan A K Tsaturyan |
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Institution: | (1) ETH Zurich, Institute for Biomechanics, HCI E 357.1, 8093 Zurich, Switzerland;(2) Present address: European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany; |
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Abstract: | The interference fine structure of the M3 reflection in the low-angle X-ray diffraction patterns of muscle fibers is used
for the measurements of axial movements of myosin heads with a precision of 0.1–0.2 nm. We have measured changes in the M3
interference profile during tension rise induced by a 5°C to 30°C temperature jump in thin bundles of contracting fibers from
rabbit skeletal muscle. Interpreting the data with a point diffractor model gives an estimate for the axial movement of the
myosin heads during force rise of less than 0.6 nm. Modifications of the point diffractor model are discussed. We show that
our experimental data can be explained by a model where myosin heads bind actin in a number of structurally different states
either stereoor non-stereo-specifically. |
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