Chromosome-encoded inducible copper resistance inPseudomonas strains |
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Authors: | Eréndira Vargas Sergio Gutiérrez Ma Elena Ambriz Carlos Cervantes |
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Institution: | (1) Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, Morelia, Mich., México |
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Abstract: | NinePseudomonas strains were selected by their high copper tolerance from a population of bacteria isolated from heavy-metal polluted zones. Copper resistance (Cu
r
) was inducible by previous exposure of cultures to subinhibitory amounts of copper sulfate. All nine strains possessed large plasmids, but transformation and curing results suggest that Cu
r
is conferred by chromosomal genes. Plasmid-lessPseudomonas aeruginosa PAO-derived strains showed the same level of Cu
r
as environmental isolates and their resistance to copper was also inducible. Total DNA from the environmentalPseudomonas, as well as fromP. aeruginosa PAO strains, showed homology to a Cu
r
P. syringae cop probe at low-stringency conditions but failed to hybridize at high-stringency conditions. |
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Keywords: | copper resistance Pseudomonas |
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