A two‐dimensional proteome map of the aflatoxigenic fungus Aspergillus flavus |
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| Authors: | Olga Pechanova Tibor Pechan Jose M Rodriguez W Paul Williams Ashli E Brown |
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| Institution: | 1. Mississippi State Chemical Laboratory, Mississippi State University, , Mississippi State, MS, USA;2. Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, , Mississippi State, MS, USA;3. US Department of Agriculture, Agricultural Research Services, Corn Host Plant Resistance Research Unit, , Mississippi State, MS, USA;4. Department of Biochemistry, Molecular Biology, Entomology, and Pant Pathology, Mississippi State University, , Mississippi State, MS, USA |
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| Abstract: | The filamentous fungus Aspergillus flavus is an opportunistic soil‐borne pathogen that produces aflatoxins, the most potent naturally occurring carcinogenic compounds known. This work represents the first gel‐based profiling analysis of A. flavus proteome and establishes a 2D proteome map. Using 2DE and MALDI‐TOF‐MS/MS, we identified 538 mycelial proteins of the aflatoxigenic strain NRRL 3357, the majority of which were functionally annotated as related to various cellular metabolic and biosynthetic processes. Additionally, a few enzymes from the aflatoxin synthesis pathway were also identified. |
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| Keywords: | 2DE Aflatoxin Aspergillus flavus Microbiology Proteome |
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