| 盾叶薯蓣内生真菌Dzf13抗细菌活性成分 |
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| 引用本文： | 蔡晓月,李培琴,单体江,徐利剑,胥岩,周立刚.盾叶薯蓣内生真菌Dzf13抗细菌活性成分[J].天然产物研究与开发,2010,22(2). |
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| 作者姓名： | 蔡晓月 李培琴 单体江 徐利剑 胥岩 周立刚 |
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| 作者单位： | 中国农业大学农学与生物技术学院,北京,100193 |
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| 基金项目： | 北京市自然科学基金，国家自然科学基金 |
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| 摘 要： | 采用活性追踪分离的方法,从盾叶薯蓣内生真菌菌株Dzf13中分离到两个具抗细菌活性的化合物1和2,经理化和波谱分析鉴定为麦角甾-5,7,22-三烯-3β-醇(1)和5α,8α-过氧麦角甾-6,22-二烯-3β-醇(2).结果表明,化合物1和2对根癌土壤杆菌、黄瓜角斑病菌和番茄疮痂病菌等三种植物病原细菌的生长均表现出一定的抑制活性,尤其对黄瓜角斑病菌的抑制活性较强.结果还表明5α,8α-过氧麦角甾-6,22-二烯-3β-醇(2)的抗细菌活性要比麦角甾-5,7,22-三烯-3β-醇(1)强.
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| 关 键 词： | 盾叶薯蓣 内生真菌Dzf13 抗细菌活性成分 抗细菌活性 |
Antibacterial Compounds from the Endophytic Fungal Isolate Dzf13 Associated with Dioscorea zingiberensis |
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| CAI Xiao-yue,LI Pei-qin,SHAN Ti-jiang,XU Li-jian,XU Yan,ZHOU Li-gang.Antibacterial Compounds from the Endophytic Fungal Isolate Dzf13 Associated with Dioscorea zingiberensis[J].Natural Product Research and Development,2010,22(2). |
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| Authors: | CAI Xiao-yue LI Pei-qin SHAN Ti-jiang XU Li-jian XU Yan ZHOU Li-gang |
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| Institution: | CAI Xiao-yue,LI Pei-qin,SHAN Ti-jiang,XU Li-jian,XU Yan,ZHOU Li-gangCollege of Agronomy , Biotechnology,China Agricultural University,Beijing 100193,China |
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| Abstract: | Two antibacterial compounds,ergosta-5,7,22-trien-3β-ol and 5α,8α-epidioxyergosta-6,22-dien-3β-ol,were isolated and identified by bioassay-guided fractionation from the extract of the endophytic fungal isolate Dzf13 from the rhizomes of Dioscorea zingiberensis,a dioscoreaceous medicinal plant.Both two compounds were screened and have inhibitory activity on plant pathogenic bacteria such as Agrobacterium tumefaciens,Pseudomonas lachrymans and Xanthomonas vesicatoria by using the microplate-MTT colorimetric assay.Of three bacterial pathogens.P.lacrymans waS most effectively inhibited.In addition,5α,8α-epidloxyergosta-6,22-dien-3β-ol was found to be more bioactive than ergosta-5,7,22-trien-3β-ol. |
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| Keywords: | Dioscorea zingiberensis endopbytic fungal isolate Dzf13 antibacterial compounds antibacterial activity |
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