Fusiogenic endogenous-retroviral syncytin-1 exerts anti-apoptotic functions in staurosporine-challenged CHO cells |
| |
Authors: | Ina Knerr Markus Schnare Kathrin Hermann Susanne Kausler Manfred Lehner Tina Vogler Wolfgang Rascher Udo Meißner |
| |
Institution: | (1) Children and Youth Hospital, University of Erlangen-Nuremberg, Loschgestr. 15, D-91054 Erlangen, Germany;(2) Institute for Clinical Microbiology, Immunology and Hygiene, University of Erlangen-Nuremberg, Erlangen, Germany;(3) Centre for Children’s Health, St. Bernward Hospital, Hildesheim, Germany |
| |
Abstract: | Fusiogenic glycoprotein syncytin-1, expressed in human placenta, is a promising candidate for acquiring a basic knowledge
of placental syncytialization. However, its cellular mode of action is unidentified. We investigated whether syncytin-1 may
exert influence on apoptotic processes. Therefore, we incubated CHO cells after stable transfection with syncytin-1 (CHO-52)
in the presence or absence of staurosporine (STS), a kinase inhibitor well characterized to induce apoptosis. When testing
the phenotype of CHO-52 cells, we could demonstrate that the induction of apoptosis by STS was delayed over a period of up
to 24 h. Furthermore, the cell death rate was decreased by approx 75% following transfection of syncytin-1 in CHO-52 compared
to mock-treated cells. In detail, after 18h of incubation with 500 nM STS, 64 ± 2% of CHO-52 cells were viable compared to
16 ± 1% of CHO-mocks, after 24 h 43 ± 3% vs 5 ± 2%, respectively. CHO-52 cells exhibited a lower expression of active caspase
3 and anti-apoptotic Bcl-2 was found to be increased in CHO-52 cells at baseline and following STS treatment.
Our study provides first evidence that syncytin-1 serves anti-apoptotic function under certain conditions. A lessened activation
of caspase 3 and an increased expression of Bcl-2 are possible mechanisms. |
| |
Keywords: | Apoptosis Bcl-2 Caspase 3 CHO Staurosporine Syncytin-1 |
本文献已被 PubMed SpringerLink 等数据库收录! |
|