尿酸酶－鲁米诺化学发光传感器

研制了依赖于鲁米诺化学发光反应和固定化尿酸酶柱的测定血清尿酸的生物传感器。其测定血清样品响应时间47s。测定每份样品需时1．5min，样品体积17μl。工作曲线的线性范围1~20mg／dl。批内不精密度3．22％～4.36％,批间6.18%～7．8%。测定值回收率为93％～109％。与医院常规酶试剂盘方法比较相关系数r=0.9909。固定化尿酸酶柱室温使用，4℃冰箱保存，连续使用5个半月测定样品2000次以上，仍保持原酶柱活力的94%。

Biosensor Based on Chemiluminescence for Serum Uric Acid Determination

A biosensor based on luminol chemiluminescent reaction and immobilized uricase column for serum uric acid determination has been developed. The response time by the sensor for various concentration of serum uric acid was 47 seconds with 17ul sample volume and at 40 samples per hour. The linear range of standard curve was from 1 up to 20 mg/dl of serum uric acid. The within day imprecision was 3.22%~4. 36%. The day to day imprecision was 6.18%-7.80%. The recovery rate by the method was 93% ~109%. Comparised with standard colorimetric method employed enzymatic kit revealed that the linear regression and correlation coerricient were Y=0. 4+0. 938X and r = 0. 9909 respectively. The immobilized uricase column retained 94% of its original activity even after over 2000 runs for five and half monthes' continual usage.